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A\nprevious study showed that the lightharvesting-\nreaction center core complex (LH1-\nRC) purified from this bacterium is highly\nstable at room temperature [Suzuki et al.,\n(2007) Biochim. Biophys. Acta 1767, 1057-1063].\nIn this work, we demonstrate that thermal\nstability of the Tch. tepidum LH1-RC is much\nhigher than that of its mesophilic counterparts\nand the enhanced thermal stability requires\nCa2+ as a cofactor. Removal of the Ca2+ from\nTch. tepidum LH1-RC resulted in a complex\nwith the same degree of thermal stability to\nthat of the LH1-RCs purified from mesophilic\nbacteria. The enhanced thermal stability can\nbe restored by addition of Ca2+ to the Ca2+-\ndepleted LH1-RC, and this process is fully\nreversible. Interchange of the thermal\nstability between the two forms is accompanied\nby a shift of the LH1 Qy transition between 915\nnm for the native and 880 nm for the Ca2+-\ndepleted LH1-RC. Differential scanning\ncalorimetry measurements reveal that\ndegradation temperature of the native LH1-RC\nis 15 °C higher and the enthalpy change is\nabout 28% larger than the Ca2+-depleted LH1-\nRC. Substitution of the Ca2+ with other metal\ncations caused a decrease in thermal stability of\nextent depending on the properties of the\ncations. These results indicate that Ca2+ ions\nplay a dual role in stabilizing the structure of\nthe pigment-membrane protein complex and\naltering its spectroscopic properties, and hence\nprovide insight into the adaptive strategy of\nthis photosynthetic organism to survive in\nextreme environments using natural resources.", "subitem_description_type": "Other"}]}, "item_6_identifier_registration": {"attribute_name": "ID登録", "attribute_value_mlt": [{"subitem_identifier_reg_text": "10.34405/00010244", "subitem_identifier_reg_type": "JaLC"}]}, "item_6_publisher_35": {"attribute_name": "出版者", "attribute_value_mlt": [{"subitem_publisher": "American Society for Biochemistry and Molecular Biology"}]}, "item_6_text_1": {"attribute_name": "Title in Japanese", "attribute_value_mlt": [{"subitem_text_value": "Calcium ions are required for the enhanced thermal stability of the light-harvesting-reaction center core complex from thermophilic purple sulfur bacterium Thermochromatium tepidum"}]}, "item_6_text_17": {"attribute_name": "形態", "attribute_value_mlt": [{"subitem_text_value": "737875 bytes"}]}, "item_6_text_47": {"attribute_name": "資源タイプ・ローカル", "attribute_value_mlt": [{"subitem_text_value": "雑誌掲載論文"}]}, "item_6_text_48": {"attribute_name": "資源タイプ・NII", "attribute_value_mlt": [{"subitem_text_value": "Journal Article"}]}, "item_6_text_49": {"attribute_name": "資源タイプ・DCMI", "attribute_value_mlt": [{"subitem_text_value": "text"}]}, "item_6_text_50": {"attribute_name": "資源タイプ・ローカル表示コード", "attribute_value_mlt": [{"subitem_text_value": "09"}]}, "item_6_text_76": {"attribute_name": "URI", "attribute_value_mlt": [{"subitem_text_value": "http://hdl.handle.net/10109/1420"}]}, "item_6_text_79": {"attribute_name": "コメント", "attribute_value_mlt": [{"subitem_text_value": "This research was originally published in The Journal of Biological Chemistry. Yukihiro Kimura, Long-Jiang Yu, Yu Hirano, Hiroaki Suzuki, and Zheng-Yu Wang. Calcium Ions Are Required for the Enhanced Thermal Stability of the Light-\nHarvesting-Reaction Center Core Complex from Thermophilic Purple Sulfur\nBacterium Thermochromatium tepidum. The Journal of Biological Chemistry. 2009. Vol.234:p.93-99. © the American Society for Biochemistry and Molecular Biology"}]}, "item_6_version_type_18": {"attribute_name": "著者版フラグ", "attribute_value_mlt": [{"subitem_version_resource": "http://purl.org/coar/version/c_ab4af688f83e57aa", "subitem_version_type": "AM"}]}, "item_creator": {"attribute_name": "著者", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "KIMURA, Yukihiro / YU, Long Jiang / HIRANO, Yu / SUZUKI, Hiroaki / WANG, Zheng Yu"}], "nameIdentifiers": [{"nameIdentifier": "21817", "nameIdentifierScheme": "WEKO"}]}]}, "item_files": {"attribute_name": "ファイル情報", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2020-02-21"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "20100095.pdf", "filesize": [{"value": "737.9 kB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_free", "mimetype": "application/pdf", "size": 737900.0, "url": {"label": "20100095.pdf", "url": "https://rose-ibadai.repo.nii.ac.jp/record/10250/files/20100095.pdf"}, "version_id": "a4ae232c-f22d-42b1-b455-1dfdec360186"}]}, "item_language": {"attribute_name": "言語", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"resourcetype": "journal article", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "Calcium ions are required for the enhanced thermal stability of the light-harvesting-reaction center core complex from thermophilic purple sulfur bacterium Thermochromatium tepidum", "item_titles": {"attribute_name": "タイトル", "attribute_value_mlt": [{"subitem_title": "Calcium ions are required for the enhanced thermal stability of the light-harvesting-reaction center core complex from thermophilic purple sulfur bacterium Thermochromatium tepidum"}]}, "item_type_id": "6", "owner": "1", "path": ["321"], "permalink_uri": "https://doi.org/10.34405/00010244", "pubdate": {"attribute_name": "公開日", "attribute_value": "2010-07-02"}, "publish_date": "2010-07-02", "publish_status": "0", "recid": "10250", "relation": {}, "relation_version_is_last": true, "title": ["Calcium ions are required for the enhanced thermal stability of the light-harvesting-reaction center core complex from thermophilic purple sulfur bacterium Thermochromatium tepidum"], "weko_shared_id": -1}
Calcium ions are required for the enhanced thermal stability of the light-harvesting-reaction center core complex from thermophilic purple sulfur bacterium Thermochromatium tepidum
https://doi.org/10.34405/00010244
https://doi.org/10.34405/0001024436199fa9-120c-49a7-9f48-4fe746c1ba02
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20100095.pdf (737.9 kB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||||
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公開日 | 2010-07-02 | |||||||
タイトル | ||||||||
タイトル | Calcium ions are required for the enhanced thermal stability of the light-harvesting-reaction center core complex from thermophilic purple sulfur bacterium Thermochromatium tepidum | |||||||
言語 | ||||||||
言語 | eng | |||||||
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資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||||
資源タイプ | journal article | |||||||
ID登録 | ||||||||
ID登録 | 10.34405/00010244 | |||||||
ID登録タイプ | JaLC | |||||||
Title in Japanese | ||||||||
Calcium ions are required for the enhanced thermal stability of the light-harvesting-reaction center core complex from thermophilic purple sulfur bacterium Thermochromatium tepidum | ||||||||
著者 |
KIMURA, Yukihiro / YU, Long Jiang / HIRANO, Yu / SUZUKI, Hiroaki / WANG, Zheng Yu
× KIMURA, Yukihiro / YU, Long Jiang / HIRANO, Yu / SUZUKI, Hiroaki / WANG, Zheng Yu
WEKO
21817
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内容記述 | ||||||||
内容記述タイプ | Other | |||||||
内容記述 | Thermochromatium (Tch.) tepidum is a thermophilic purple sulfur photosynthetic bacterium collected from the Mammoth Hot Springs, Yellowstone National Park. A previous study showed that the lightharvesting- reaction center core complex (LH1- RC) purified from this bacterium is highly stable at room temperature [Suzuki et al., (2007) Biochim. Biophys. Acta 1767, 1057-1063]. In this work, we demonstrate that thermal stability of the Tch. tepidum LH1-RC is much higher than that of its mesophilic counterparts and the enhanced thermal stability requires Ca2+ as a cofactor. Removal of the Ca2+ from Tch. tepidum LH1-RC resulted in a complex with the same degree of thermal stability to that of the LH1-RCs purified from mesophilic bacteria. The enhanced thermal stability can be restored by addition of Ca2+ to the Ca2+- depleted LH1-RC, and this process is fully reversible. Interchange of the thermal stability between the two forms is accompanied by a shift of the LH1 Qy transition between 915 nm for the native and 880 nm for the Ca2+- depleted LH1-RC. Differential scanning calorimetry measurements reveal that degradation temperature of the native LH1-RC is 15 °C higher and the enthalpy change is about 28% larger than the Ca2+-depleted LH1- RC. Substitution of the Ca2+ with other metal cations caused a decrease in thermal stability of extent depending on the properties of the cations. These results indicate that Ca2+ ions play a dual role in stabilizing the structure of the pigment-membrane protein complex and altering its spectroscopic properties, and hence provide insight into the adaptive strategy of this photosynthetic organism to survive in extreme environments using natural resources. |
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書誌情報 |
The Journal of Biological Chemistry 号 284, p. 93-99, 発行日 2009-01-02 |
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内容記述タイプ | Other | |||||||
内容記述 | application/pdf | |||||||
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出版タイプ | AM | |||||||
出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa | |||||||
出版者 | ||||||||
出版者 | American Society for Biochemistry and Molecular Biology | |||||||
資源タイプ | ||||||||
内容記述タイプ | Other | |||||||
内容記述 | 論文(Article) | |||||||
資源タイプ・NII | ||||||||
Journal Article | ||||||||
資源タイプ・DCMI | ||||||||
text |