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Calcium ions are required for the enhanced thermal stability of the light-harvesting-reaction center core complex from thermophilic purple sulfur bacterium Thermochromatium tepidum
https://doi.org/10.34405/00010244
https://doi.org/10.34405/0001024436199fa9-120c-49a7-9f48-4fe746c1ba02
| 名前 / ファイル | ライセンス | アクション |
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20100095.pdf (737.9 kB)
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| Item type | 学術雑誌論文 / Journal Article(1) | |||||||
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| 公開日 | 2010-07-02 | |||||||
| タイトル | ||||||||
| タイトル | Calcium ions are required for the enhanced thermal stability of the light-harvesting-reaction center core complex from thermophilic purple sulfur bacterium Thermochromatium tepidum | |||||||
| 言語 | ||||||||
| 言語 | eng | |||||||
| 資源タイプ | ||||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||||
| 資源タイプ | journal article | |||||||
| ID登録 | ||||||||
| ID登録 | 10.34405/00010244 | |||||||
| ID登録タイプ | JaLC | |||||||
| Title in Japanese | ||||||||
| Calcium ions are required for the enhanced thermal stability of the light-harvesting-reaction center core complex from thermophilic purple sulfur bacterium Thermochromatium tepidum | ||||||||
| 著者 |
KIMURA, Yukihiro / YU, Long Jiang / HIRANO, Yu / SUZUKI, Hiroaki / WANG, Zheng Yu
× KIMURA, Yukihiro / YU, Long Jiang / HIRANO, Yu / SUZUKI, Hiroaki / WANG, Zheng Yu
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| 内容記述 | ||||||||
| 内容記述タイプ | Other | |||||||
| 内容記述 | Thermochromatium (Tch.) tepidum is a thermophilic purple sulfur photosynthetic bacterium collected from the Mammoth Hot Springs, Yellowstone National Park. A previous study showed that the lightharvesting- reaction center core complex (LH1- RC) purified from this bacterium is highly stable at room temperature [Suzuki et al., (2007) Biochim. Biophys. Acta 1767, 1057-1063]. In this work, we demonstrate that thermal stability of the Tch. tepidum LH1-RC is much higher than that of its mesophilic counterparts and the enhanced thermal stability requires Ca2+ as a cofactor. Removal of the Ca2+ from Tch. tepidum LH1-RC resulted in a complex with the same degree of thermal stability to that of the LH1-RCs purified from mesophilic bacteria. The enhanced thermal stability can be restored by addition of Ca2+ to the Ca2+- depleted LH1-RC, and this process is fully reversible. Interchange of the thermal stability between the two forms is accompanied by a shift of the LH1 Qy transition between 915 nm for the native and 880 nm for the Ca2+- depleted LH1-RC. Differential scanning calorimetry measurements reveal that degradation temperature of the native LH1-RC is 15 °C higher and the enthalpy change is about 28% larger than the Ca2+-depleted LH1- RC. Substitution of the Ca2+ with other metal cations caused a decrease in thermal stability of extent depending on the properties of the cations. These results indicate that Ca2+ ions play a dual role in stabilizing the structure of the pigment-membrane protein complex and altering its spectroscopic properties, and hence provide insight into the adaptive strategy of this photosynthetic organism to survive in extreme environments using natural resources. |
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| 書誌情報 |
The Journal of Biological Chemistry 号 284, p. 93-99, 発行日 2009-01-02 |
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| 内容記述タイプ | Other | |||||||
| 内容記述 | application/pdf | |||||||
| 著者版フラグ | ||||||||
| 出版タイプ | AM | |||||||
| 出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa | |||||||
| 出版者 | ||||||||
| 出版者 | American Society for Biochemistry and Molecular Biology | |||||||
| 資源タイプ | ||||||||
| 内容記述タイプ | Other | |||||||
| 内容記述 | 論文(Article) | |||||||
| 資源タイプ・NII | ||||||||
| Journal Article | ||||||||
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