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We demonstrate in this study,\nusing a highly purified LH1-reaction center\ncomplex, that the LH1 Qy transition is strongly\ndependent on metal cations and Ca2+ is\ninvolved in the unusual red-shift. Removal of\nthe Ca2+ resulted in formation of a species with\nthe LH1 Qy absorption at 880 nm, and addition\nof the Ca2+ to the 880-nm species recovered the\nnative 915-nm form. Interchange between the\ntwo forms is fully reversible. Based on\nspectroscopic and isothermal titration\ncalorimetry analyses, the Ca2+-binding to the\nLH1 complex was estimated to occur in a\nstoichiometric ratio of Ca2+/αβ -subunit = 1:1\nand the binding constant was in 105 M-1 order of\nmagnitude, which is comparable with those for\nEF-hand Ca2+-binding proteins. Despite the\nhigh affinity, conformational changes in the\nLH1 complex upon Ca2+-binding were small\nand occurred slowly with a typical time\nconstant of about 6 minutes. Replacement of\nthe Ca2+ with other metal cations caused blueshifts\nof the Qy bands depending on the\nproperty of the cations, indicating that the\nbinding site is highly selective. Based on the\namino acid sequences of the LH1 complex,\npossible Ca2+-binding sites are proposed, which\nconsists of several acidic amino acid residues\nnear the membrane interfaces of the Cterminal\nregion of the α-polypeptide and the Nterminal\nregion of the β-polypeptide.", "subitem_description_type": "Other"}]}, "item_6_identifier_registration": {"attribute_name": "ID登録", "attribute_value_mlt": [{"subitem_identifier_reg_text": "10.34405/00010245", "subitem_identifier_reg_type": "JaLC"}]}, "item_6_publisher_35": {"attribute_name": "出版者", "attribute_value_mlt": [{"subitem_publisher": "American Society for Biochemistry and Molecular Biology"}]}, "item_6_text_1": {"attribute_name": "Title in Japanese", "attribute_value_mlt": [{"subitem_text_value": "Calcium Ions Are Involved in the Unusual Red-Shift of the Light-Harvesting 1 Qy Transition of the Core Complex in Thermophilic Purple Sulfur Bacterium Thermochromatium tepidum"}]}, "item_6_text_17": {"attribute_name": "形態", "attribute_value_mlt": [{"subitem_text_value": "995727 bytes"}]}, "item_6_text_47": {"attribute_name": "資源タイプ・ローカル", "attribute_value_mlt": [{"subitem_text_value": "雑誌掲載論文"}]}, "item_6_text_48": {"attribute_name": "資源タイプ・NII", "attribute_value_mlt": [{"subitem_text_value": "Journal Article"}]}, "item_6_text_49": {"attribute_name": "資源タイプ・DCMI", "attribute_value_mlt": [{"subitem_text_value": "text"}]}, "item_6_text_50": {"attribute_name": "資源タイプ・ローカル表示コード", "attribute_value_mlt": [{"subitem_text_value": "09"}]}, "item_6_text_76": {"attribute_name": "URI", "attribute_value_mlt": [{"subitem_text_value": "http://hdl.handle.net/10109/1421"}]}, "item_6_text_79": {"attribute_name": "コメント", "attribute_value_mlt": [{"subitem_text_value": "This research was originally published in The Journal of Biological Chemistry. Yukihiro Kimura, Yu Hirano, Long-Jiang Yu, Hiroaki Suzuki, Masayuki Kobayashi, and\nZheng-Yu Wang. Calcium Ions Are Involved in the Unusual Red-Shift of the Light-Harvesting 1 Qy\nTransition of the Core Complex in Thermophilic Purple Sulfur Bacterium\nThermochromatium tepidum.The Journal of Biological Chemistry. 2008. Vol.283:p.13867-13873. © the American Society for Biochemistry and Molecular Biology"}]}, "item_6_version_type_18": {"attribute_name": "著者版フラグ", "attribute_value_mlt": [{"subitem_version_resource": "http://purl.org/coar/version/c_ab4af688f83e57aa", "subitem_version_type": "AM"}]}, "item_creator": {"attribute_name": "著者", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "KIMURA, Yukihiro / HIRANO, Yu / YU, Long Jiang / SUZUKI, Hiroaki / KOBAYASHI, Masayuki / WANG, Zheng Yu"}], "nameIdentifiers": [{"nameIdentifier": "21818", "nameIdentifierScheme": "WEKO"}]}]}, "item_files": {"attribute_name": "ファイル情報", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2020-02-21"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "20100096.pdf", "filesize": [{"value": "995.7 kB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_free", "mimetype": "application/pdf", "size": 995700.0, "url": {"label": "20100096.pdf", "url": "https://rose-ibadai.repo.nii.ac.jp/record/10251/files/20100096.pdf"}, "version_id": "2dbecd80-050a-4136-a4bc-2ef793d72417"}]}, "item_language": {"attribute_name": "言語", "attribute_value_mlt": [{"subitem_language": "jpn"}]}, "item_resource_type": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"resourcetype": "journal article", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "Calcium Ions Are Involved in the Unusual Red-Shift of the Light-Harvesting 1 Qy Transition of the Core Complex in Thermophilic Purple Sulfur Bacterium Thermochromatium tepidum", "item_titles": {"attribute_name": "タイトル", "attribute_value_mlt": [{"subitem_title": "Calcium Ions Are Involved in the Unusual Red-Shift of the Light-Harvesting 1 Qy Transition of the Core Complex in Thermophilic Purple Sulfur Bacterium Thermochromatium tepidum"}]}, "item_type_id": "6", "owner": "1", "path": ["321"], "permalink_uri": "https://doi.org/10.34405/00010245", "pubdate": {"attribute_name": "公開日", "attribute_value": "2010-07-02"}, "publish_date": "2010-07-02", "publish_status": "0", "recid": "10251", "relation": {}, "relation_version_is_last": true, "title": ["Calcium Ions Are Involved in the Unusual Red-Shift of the Light-Harvesting 1 Qy Transition of the Core Complex in Thermophilic Purple Sulfur Bacterium Thermochromatium tepidum"], "weko_shared_id": -1}
Calcium Ions Are Involved in the Unusual Red-Shift of the Light-Harvesting 1 Qy Transition of the Core Complex in Thermophilic Purple Sulfur Bacterium Thermochromatium tepidum
https://doi.org/10.34405/00010245
https://doi.org/10.34405/0001024508e11cbb-51db-46f0-916a-5f911d4f65a1
名前 / ファイル | ライセンス | アクション |
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20100096.pdf (995.7 kB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||||
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公開日 | 2010-07-02 | |||||||
タイトル | ||||||||
タイトル | Calcium Ions Are Involved in the Unusual Red-Shift of the Light-Harvesting 1 Qy Transition of the Core Complex in Thermophilic Purple Sulfur Bacterium Thermochromatium tepidum | |||||||
言語 | ||||||||
言語 | jpn | |||||||
資源タイプ | ||||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||||
資源タイプ | journal article | |||||||
ID登録 | ||||||||
ID登録 | 10.34405/00010245 | |||||||
ID登録タイプ | JaLC | |||||||
Title in Japanese | ||||||||
Calcium Ions Are Involved in the Unusual Red-Shift of the Light-Harvesting 1 Qy Transition of the Core Complex in Thermophilic Purple Sulfur Bacterium Thermochromatium tepidum | ||||||||
著者 |
KIMURA, Yukihiro / HIRANO, Yu / YU, Long Jiang / SUZUKI, Hiroaki / KOBAYASHI, Masayuki / WANG, Zheng Yu
× KIMURA, Yukihiro / HIRANO, Yu / YU, Long Jiang / SUZUKI, Hiroaki / KOBAYASHI, Masayuki / WANG, Zheng Yu
WEKO
21818
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内容記述 | ||||||||
内容記述タイプ | Other | |||||||
内容記述 | Thermophilic purple sulfur bacterium, Thermochromatium tepidum, can grow at temperatures up to 58 °C and exhibits an unusual Qy absorption at 915 nm for the core light-harvesting complex (LH1), about 35 nm red-shift from those of its mesophilic counterparts. We demonstrate in this study, using a highly purified LH1-reaction center complex, that the LH1 Qy transition is strongly dependent on metal cations and Ca2+ is involved in the unusual red-shift. Removal of the Ca2+ resulted in formation of a species with the LH1 Qy absorption at 880 nm, and addition of the Ca2+ to the 880-nm species recovered the native 915-nm form. Interchange between the two forms is fully reversible. Based on spectroscopic and isothermal titration calorimetry analyses, the Ca2+-binding to the LH1 complex was estimated to occur in a stoichiometric ratio of Ca2+/αβ -subunit = 1:1 and the binding constant was in 105 M-1 order of magnitude, which is comparable with those for EF-hand Ca2+-binding proteins. Despite the high affinity, conformational changes in the LH1 complex upon Ca2+-binding were small and occurred slowly with a typical time constant of about 6 minutes. Replacement of the Ca2+ with other metal cations caused blueshifts of the Qy bands depending on the property of the cations, indicating that the binding site is highly selective. Based on the amino acid sequences of the LH1 complex, possible Ca2+-binding sites are proposed, which consists of several acidic amino acid residues near the membrane interfaces of the Cterminal region of the α-polypeptide and the Nterminal region of the β-polypeptide. |
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書誌情報 |
The Journal of Biological Chemistry 号 283, p. 13867-13873, 発行日 2008-05-16 |
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内容記述タイプ | Other | |||||||
内容記述 | application/pdf | |||||||
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出版タイプ | AM | |||||||
出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa | |||||||
出版者 | ||||||||
出版者 | American Society for Biochemistry and Molecular Biology | |||||||
資源タイプ | ||||||||
内容記述タイプ | Other | |||||||
内容記述 | 論文(Article) | |||||||
資源タイプ・NII | ||||||||
Journal Article | ||||||||
資源タイプ・DCMI | ||||||||
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